Chromatographic Separation

Chromatography basically involves the separation of mixtures due to differences in the distribution coëfficient of sample components between two different phases.

One of these phases is a mobile phase and the other is a stationary phase.

Different affinity of these components to stationary phase causes the separation.

Kinds of chromatography :

          Liquid column chromatography
          Gas liquid chromatography
          Thin layer chromatography

          Ion Exchange chromatography

Separation in ion exchange chromatography is based on the competition of different ionic compounds of the sample for the active sites on the ion exchange resin.

Applicable in following processes :

          Beet and cane molasses sucrose recovery
          Biomass hydrolysate treatment to recycle acid values
          Mannitol - sorbitol recovery after dextrose hydrogenation
          Xylose - glucose
          Xylitol purification
          Fructose enrichment to produce HFSC ( high fructose ) for soft drinks
          High purity dextrose production
          Polysaccharide isolation and use in pharmaceutical applications
          Fermentation product treatment for isolation
          Betaine recovery from beet molasses
          Organic acid isolation from cane processing by-products